FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-50_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-50_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23789673
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC372100.15641240634118844No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC273870.11512138060914079No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG255440.10737432162266375No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC252840.10628141042543963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG57200.039.80467615
CTCGTAT42050.039.29484642
TCGTATG51400.038.59013743
TATGCCG53200.037.15593746
ACTGACC55750.035.76356532
TGTTACC81450.034.26472513
CACGTCT67550.033.65487714
ACACGTC69300.033.2033913
TTACCTC83700.033.17848615
AGTCACT61100.033.1403828
CGTATGC60400.032.8977544
TCTAGTT97300.031.5887747
TTTTTCC157050.031.3502251
TAGTTGT96050.029.7387549
CTCTAGT104550.029.6954566
CTAATGC94600.029.1712421
TCTAATG95100.029.09041420
GTATGCC68750.028.90229245
CAGTCAC72600.028.50855627
ACTCTAG112550.027.6764355
Produced by FastQC (version 0.11.2)