FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-49_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-49_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22408365
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC298820.13335198708160992No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG266880.11909838134107509No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG262140.11698309983793999No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG257320.11483211738116546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG40700.034.1584315
TCGTATG33700.033.88183243
CTCGTAT34350.032.135842
TATGCCG37050.030.91257346
CACGTCT48050.028.93368314
AGTCACG42550.028.1312528
TATCTCG39800.027.21507639
GTCACGC44750.026.82534629
CGTATGC43700.026.28707744
CTTAAAC56050.025.8544641
CTATAAG82950.025.6182646
GTCGCTA52100.025.49072327
CGCTATG51850.025.34751129
ACACGTC56500.025.27824413
ACCTATA57400.023.9788786
TATAAGC89400.023.7314847
GTATGCC51200.022.9757545
CAGTCAC53850.022.67653527
GCCAATA51650.022.64052834
CGCCAAT53150.022.26117333
Produced by FastQC (version 0.11.2)