FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-48_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-48_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9593548
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG164310.17127135862560963RNA PCR Primer, Index 28 (100% over 43bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC101790.10610255976204008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25600.050.80042642
TCGTATG28200.049.90931743
TATGCCG29300.047.33041446
ACGTCTG32150.046.13888515
GTATGCC34000.041.19352345
ACACGTC36600.041.00122513
CACGTCT36650.040.7568214
CAGTCAC38550.037.85120827
ATGCCGT37400.037.63333547
CGTATGC38150.036.893344
GTCTGAA42700.035.2226817
AGTCACC44550.032.13395328
GCCGTCT44200.031.68749449
CCAGTCA47450.030.75161426
CCGTCTT44900.030.6556650
CACACGT51300.029.25218412
TGTTACC24900.028.67871313
AGATCTC46350.028.2067638
TGCCGTC51900.027.45154848
TCCAGTC54050.027.18802525
Produced by FastQC (version 0.11.2)