FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-45_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-45_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63461173
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG1046140.16484725235066172No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG780370.12296810208660972No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC707420.11147288437293776No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG687660.10835916947201717No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC667810.10523127267124419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG133950.044.114843
CTCGTAT118500.043.54885542
ACGTCTG157500.041.9223315
TATGCCG146650.040.27229746
CACGTCT181900.036.69778414
CGTATGC174000.033.9419544
AGTCACA182200.033.3781828
CAGTCAC183800.033.36913727
ACACGTC206300.032.80905513
GTATGCC186700.031.91038745
TGTTACC151000.029.7459613
ATGCCGT202300.029.60333347
GTCACAT209700.028.65552929
TCTAGTT181050.027.2267727
GTCTGAA249550.026.83058417
CTAATGC166600.026.2749321
TTTTTCC307950.026.2069511
CTATAAG314350.026.0416846
TAGTTGT179250.025.7120179
CCAGTCA246300.025.40572526
Produced by FastQC (version 0.11.2)