FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-44_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-44_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41705785
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC519370.12453188448556957No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG445870.10690843008949477No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT438320.10509812967193878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG76350.036.95958315
CTCGTAT61000.034.1559242
TCGTATG68800.033.99440443
GTCACAC76000.033.4054929
TATGCCG74850.032.21570646
ACACGTC89950.031.8709613
CGTATGC74450.031.73993744
CACGTCT91350.030.96669414
CGCTATG113450.029.24988729
GTCGCTA116850.028.48736427
CTTAAAC122650.027.9341051
AGTCACA92250.027.85758828
TGTTACC89000.026.97829413
TCACACT96450.026.57297530
ACCTATA131450.026.268356
TTACCTC91300.025.95785115
CAGTCAC101500.025.65869127
GGTAACT131850.024.76076769
GTATGCC99100.024.61089945
TATAACC140300.024.5146689
Produced by FastQC (version 0.11.2)