FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-41_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-41_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8771053
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG130780.14910410414804243No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG127920.14584337821239934RNA PCR Primer, Index 32 (100% over 43bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC110570.1260624009454737No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG92250.10517551313394184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21550.051.38232442
TCGTATG24100.050.38329743
TATGCCG25600.048.10680846
ACGTCTG28250.045.06025315
CGTATGC28350.042.9531444
CACGTCT30100.042.4056214
ACACGTC30900.041.86618813
GTATGCC29950.041.46474545
CAGTCAC31950.039.5149327
ACTCAAT31750.038.56897735
ATGCCGT32450.037.84543647
GTCTGAA36500.035.25204517
TGTTACC25250.034.42942413
AGTCACC36500.034.4000928
TTACCTC26700.033.07610715
TAGTTGT27750.032.195879
CCGTCTT38200.032.14899450
GCCGTCT38250.032.01677749
TCTAGTT30200.031.9819267
CCACTCA39600.030.6622133
Produced by FastQC (version 0.11.2)