FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-40_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-40_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9200975
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA571980.6216515097584767TruSeq Adapter, Index 27 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC135890.14769086971761144No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG117330.12751909444379536No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG107510.11684631248318793No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA93300.10140229703917247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT73700.062.62793444
TCGTATG78950.062.04626545
TATGCCG81500.060.31657848
ACGTCTG86800.058.74035315
GTATGCC84200.058.38243547
GTCACAT85800.058.3767529
ATGCCGT85300.057.75119449
TATCTCG80050.057.7436241
AGTCACA87500.057.67622828
ACATTCC88450.056.2764532
CACGTCT90550.056.2318114
CAGTCAC88950.056.1543227
TTATCTC82650.055.92651440
ACACGTC92750.055.0842813
CACATTC91450.054.76949731
CGTATGC90200.054.498946
GCCGTCT91050.053.8379951
CCGTCTT92100.053.22477752
GTCTGAA96850.052.78483617
TGCCGTC96850.050.7215150