Basic Statistics
Measure | Value |
---|---|
Filename | HGV7YBGX2_n01_9s-40_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9200975 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 57198 | 0.6216515097584767 | TruSeq Adapter, Index 27 (97% over 44bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 13589 | 0.14769086971761144 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 11733 | 0.12751909444379536 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 10751 | 0.11684631248318793 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA | 9330 | 0.10140229703917247 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 7370 | 0.0 | 62.627934 | 44 |
TCGTATG | 7895 | 0.0 | 62.046265 | 45 |
TATGCCG | 8150 | 0.0 | 60.316578 | 48 |
ACGTCTG | 8680 | 0.0 | 58.740353 | 15 |
GTATGCC | 8420 | 0.0 | 58.382435 | 47 |
GTCACAT | 8580 | 0.0 | 58.37675 | 29 |
ATGCCGT | 8530 | 0.0 | 57.751194 | 49 |
TATCTCG | 8005 | 0.0 | 57.74362 | 41 |
AGTCACA | 8750 | 0.0 | 57.676228 | 28 |
ACATTCC | 8845 | 0.0 | 56.27645 | 32 |
CACGTCT | 9055 | 0.0 | 56.23181 | 14 |
CAGTCAC | 8895 | 0.0 | 56.15432 | 27 |
TTATCTC | 8265 | 0.0 | 55.926514 | 40 |
ACACGTC | 9275 | 0.0 | 55.08428 | 13 |
CACATTC | 9145 | 0.0 | 54.769497 | 31 |
CGTATGC | 9020 | 0.0 | 54.4989 | 46 |
GCCGTCT | 9105 | 0.0 | 53.83799 | 51 |
CCGTCTT | 9210 | 0.0 | 53.224777 | 52 |
GTCTGAA | 9685 | 0.0 | 52.784836 | 17 |
TGCCGTC | 9685 | 0.0 | 50.72151 | 50 |