FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-33_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-33_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19408401
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2027161.0444755340741363RNA PCR Primer, Index 31 (100% over 43bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG267820.13799179025618855No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG202120.1041404698923935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT264350.063.9540542
TCGTATG275100.063.75110243
TATGCCG276000.063.2197246
ACGTCTG287850.062.55876515
CGTATGC281950.062.16683644
ACGATAT285400.061.81040235
CACGTCT293850.061.25902614
TATCTCG276900.061.17939439
GTATGCC285800.061.1365845
ATGCCGT287500.060.76307347
ACACGTC298400.060.57960513
CCACGAT295000.060.48884233
CGATATC280850.060.39216636
CAGTCAC295900.060.1654427
AGTCACC301900.058.7983128
ACCACGA304800.058.72506732
GTCTGAA307600.058.5170817
ATATCTC291450.058.26687238
CCGTCTT299500.057.9820550
GCCGTCT300700.057.8199449
Produced by FastQC (version 0.11.2)