FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-07_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-07_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8427678
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC146730.17410489579692057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG104910.1244826866902129RNA PCR Primer, Index 29 (100% over 43bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG97120.11523933401347322No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC86460.1025905356137242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG19650.051.26197443
CTCGTAT17300.048.45260642
TATGCCG22100.045.73653446
ACGTCTG24200.043.4785415
ACACGTC25550.041.3174913
GTATGCC25250.040.16717545
CACGTCT26250.039.95260214
CAGTCAC28050.037.13819527
ATGCCGT28250.036.39030547
TAATCTC23750.035.4385138
CGTATGC29000.034.73502344
TAGTTGT32200.034.7117929
TGTTACC32300.034.28513713
CTAATCT24300.034.21034237
TTACCTC32450.034.0193315
GTCTGAA31750.033.6802217
TCTAGTT35100.033.216887
GCCGTCT30500.032.5741349
TTTTTCC53550.032.215541
AGTCACC35300.029.80381228
Produced by FastQC (version 0.11.2)