FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4w-48_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4w-48_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531412
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA144360.19167720475257496TruSeq Adapter, Index 7 (97% over 36bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG103850.13788915013545933No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC90970.12078744331076297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21850.048.62783444
ACGTCTG24200.047.04058515
TCGTATG24000.045.85272245
TATGCCG24050.045.61364748
CACGAGT24600.045.15176431
TATCTCG23750.044.5905841
AGTCACG25000.044.5676128
TCACGAG25100.044.5274830
ACGAGTG27000.042.16034332
ACACGTC27750.041.1476413
CACGTCT27600.040.8710414
ATGCCGT28350.039.0604949
GTCACGA28850.038.85924529
CAGTCAC29300.038.4978927
GTATGCC29350.037.4945647
CGTATGC30150.036.72851646
GGATATC29350.036.5518338
GTCTGAA32750.035.7063317
ATATCTC32050.033.3656240
TGGATAT33100.033.2444837
Produced by FastQC (version 0.11.2)