FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4w-41_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4w-41_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413711
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG131880.1567441524910946No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG117530.1396886581913736RNA PCR Primer, Index 26 (100% over 43bp)
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG94640.11248306484498932No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC85230.10129893931464962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21400.052.549642
TCGTATG23250.051.9293943
ACGTCTG26400.051.22369815
TATGCCG23600.051.1613846
ACACGTC28900.046.9127913
GTCACAT27850.046.69482829
CACGTCT29300.046.3898114
AGTCACA29500.044.43388428
GTCTGAA31650.042.9415517
CAGTCAC30650.042.8792527
ATGCCGT28500.042.6075547
CGTATGC28850.041.85144
GTATGCC29950.040.6596945
CACATGA35300.037.5237831
ACATGAG34800.037.46795732
GCCGTCT31650.037.3853549
CCGTCTT34500.034.5969750
GAGCACA42350.032.5829359
CATCTCG36300.032.40413339
CTGAACT43450.031.755619
Produced by FastQC (version 0.11.2)