FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4w-40_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4w-40_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10675091
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA246140.23057414686207361TruSeq Adapter, Index 22 (97% over 40bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG136230.1276148371943621No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC117790.1103409797630765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT33950.055.7842644
CGTAATC34750.054.99353838
TCGTATG39850.054.3641345
TATGCCG40100.053.68210248
TACGTAA40700.053.56453736
ACGTAAT41000.053.08846337
CCGTACG42300.051.2120233
ACGTCTG45550.049.22638315
GTATGCC45800.047.45329747
CAGTCAC46800.046.58239727
TAATCTC42700.045.07783540
CACGTCT50050.045.07676714
ATGCCGT47750.044.79273249
AGTCACC50400.043.73417328
ACACGTC52800.043.12163513
CGTATGC52000.041.92788746
GTCTGAA54500.041.07719817
CCGTCTT54050.039.5062652
GCCGTCT54300.039.4512451
CGTACGT56450.038.92495334
Produced by FastQC (version 0.11.2)