FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4w-33_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4w-33_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9391804
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA944811.0059941625698323TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC167890.17876224844555957No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGC136250.14507330008164565No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT123330.13131662458032559No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC123260.13124209150872398No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG106790.11370552451903809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT115100.063.418944
TCGTATG122950.063.01693745
ACGTCTG127250.062.8932315
CGTATGC124350.062.47423646
GTCACAC126950.062.19716629
TATGCCG124300.062.11049348
CACGTCT129200.061.9980514
TATCTCG119150.061.11847341
ACACGTC132500.060.74066513
AGTCACA132800.059.63913328
GTATGCC130000.059.5729447
ATGCCGT129950.059.35694549
CAGTCAC133850.058.9908927
TCACACT134500.058.6288730
ACTGATA135300.058.4099934
GCCGTCT132950.057.99099351
GTCTGAA139900.057.25319317
TATATCT128250.057.07586339
ATATCTC128950.056.76693340
CCGTCTT136000.056.6907752