Basic Statistics
Measure | Value |
---|---|
Filename | HGV7YBGX2_n01_4w-33_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9391804 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 94481 | 1.0059941625698323 | TruSeq Adapter, Index 25 (97% over 44bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 16789 | 0.17876224844555957 | No Hit |
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGC | 13625 | 0.14507330008164565 | No Hit |
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT | 12333 | 0.13131662458032559 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC | 12326 | 0.13124209150872398 | No Hit |
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG | 10679 | 0.11370552451903809 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 11510 | 0.0 | 63.4189 | 44 |
TCGTATG | 12295 | 0.0 | 63.016937 | 45 |
ACGTCTG | 12725 | 0.0 | 62.89323 | 15 |
CGTATGC | 12435 | 0.0 | 62.474236 | 46 |
GTCACAC | 12695 | 0.0 | 62.197166 | 29 |
TATGCCG | 12430 | 0.0 | 62.110493 | 48 |
CACGTCT | 12920 | 0.0 | 61.99805 | 14 |
TATCTCG | 11915 | 0.0 | 61.118473 | 41 |
ACACGTC | 13250 | 0.0 | 60.740665 | 13 |
AGTCACA | 13280 | 0.0 | 59.639133 | 28 |
GTATGCC | 13000 | 0.0 | 59.57294 | 47 |
ATGCCGT | 12995 | 0.0 | 59.356945 | 49 |
CAGTCAC | 13385 | 0.0 | 58.99089 | 27 |
TCACACT | 13450 | 0.0 | 58.62887 | 30 |
ACTGATA | 13530 | 0.0 | 58.40999 | 34 |
GCCGTCT | 13295 | 0.0 | 57.990993 | 51 |
GTCTGAA | 13990 | 0.0 | 57.253193 | 17 |
TATATCT | 12825 | 0.0 | 57.075863 | 39 |
ATATCTC | 12895 | 0.0 | 56.766933 | 40 |
CCGTCTT | 13600 | 0.0 | 56.69077 | 52 |