FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4w-07_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4w-07_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8376744
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG200260.23906663495983643RNA PCR Primer, Index 24 (100% over 43bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC113490.13548223510232615No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG87220.10412160142413329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29200.055.1681642
TCGTATG32350.054.1687843
TATGCCG33300.053.76708246
ACGTCTG36550.050.3084515
AGTCACG35800.050.2003328
GTATGCC37400.048.14926545
ACACGTC40750.045.8854913
CACGTCT40600.045.7151314
GGTAGCA41100.044.5660934
ATGCCGT40750.044.36108847
TAGCATC38250.043.01687236
CGTATGC42150.042.6408444
CAGTCAC43000.042.59689327
GTCTGAA44800.041.27138517
GTCACGG45100.040.3839929
GCCGTCT45300.039.21976549
CCGTCTT45700.038.9517450
CCAGTCA48850.037.4957326
CATCTCG46300.035.23968539
CTCCAGT53500.034.6236124
Produced by FastQC (version 0.11.2)