FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4s-48_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4s-48_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10321882
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC196740.19060477536945297No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA167860.16262538168911445TruSeq Adapter, Index 18 (97% over 40bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG137550.13326058174274807No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC127160.12319458796370662No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT109450.10603686420751564No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC109290.10588185371621184No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT107510.1041573620004569No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA104890.10161906520535693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26100.054.3221444
TCGTATG28600.053.67520545
GTCACGT29750.051.94496529
ACGTCCG30800.050.1733832
TATGCCG30950.049.04264548
TCACGTC31750.048.56419430
CGCACAT32200.047.02803837
AGTCACG33650.045.92459528
ACGTCTG35150.044.9487715
GTATGCC35350.043.23129747
ATGCCGT35100.043.14587449
CACGTCT37600.042.38793614
ACACGTC37950.042.36103413
CCGCACA36400.042.2650836
CAGTCAC37900.041.41184627
TGTTACC41350.040.4630513
TAGTTGT42400.040.0295879
TTACCTC42300.039.96072815
TCTAGTT46100.039.5078437
TTTTTCC69050.037.776161