Basic Statistics
Measure | Value |
---|---|
Filename | HGV7YBGX2_n01_4s-41_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8030651 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 13625 | 0.16966245949425518 | TruSeq Adapter, Index 21 (97% over 40bp) |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 10928 | 0.13607863173234647 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 10102 | 0.12579303969254796 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC | 9397 | 0.11701417481596449 | No Hit |
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT | 8115 | 0.10105033826024815 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTCG | 2645 | 0.0 | 56.470524 | 33 |
TCGTATG | 2610 | 0.0 | 53.795166 | 45 |
CTCGTAT | 2315 | 0.0 | 52.75167 | 44 |
ACGTTTC | 2860 | 0.0 | 52.5872 | 32 |
GTCACGT | 2895 | 0.0 | 51.95143 | 29 |
TTTCGGA | 2900 | 0.0 | 51.505016 | 35 |
CACGTTT | 3025 | 0.0 | 49.71819 | 31 |
TTCGGAA | 2995 | 0.0 | 49.295418 | 36 |
TATGCCG | 2895 | 0.0 | 49.095695 | 48 |
ACGTCTG | 3255 | 0.0 | 48.008446 | 15 |
TCGGAAT | 3140 | 0.0 | 46.68946 | 37 |
AGTCACG | 3285 | 0.0 | 45.78368 | 28 |
CACGTCT | 3470 | 0.0 | 45.233807 | 14 |
CGTATGC | 3185 | 0.0 | 44.300224 | 46 |
ACACGTC | 3590 | 0.0 | 44.202553 | 13 |
CAGTCAC | 3485 | 0.0 | 43.65112 | 27 |
ATGCCGT | 3280 | 0.0 | 43.122585 | 49 |
GTATGCC | 3395 | 0.0 | 41.560005 | 47 |
GTCTGAA | 4100 | 0.0 | 38.36524 | 17 |
GCCGTCT | 3840 | 0.0 | 35.2159 | 51 |