FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4s-41_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4s-41_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8030651
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA136250.16966245949425518TruSeq Adapter, Index 21 (97% over 40bp)
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG109280.13607863173234647No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG101020.12579303969254796No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC93970.11701417481596449No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT81150.10105033826024815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG26450.056.47052433
TCGTATG26100.053.79516645
CTCGTAT23150.052.7516744
ACGTTTC28600.052.587232
GTCACGT28950.051.9514329
TTTCGGA29000.051.50501635
CACGTTT30250.049.7181931
TTCGGAA29950.049.29541836
TATGCCG28950.049.09569548
ACGTCTG32550.048.00844615
TCGGAAT31400.046.6894637
AGTCACG32850.045.7836828
CACGTCT34700.045.23380714
CGTATGC31850.044.30022446
ACACGTC35900.044.20255313
CAGTCAC34850.043.6511227
ATGCCGT32800.043.12258549
GTATGCC33950.041.56000547
GTCTGAA41000.038.3652417
GCCGTCT38400.035.215951