FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4s-40_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4s-40_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8556149
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC327800.3831162828043317No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG257930.30145571331214543RNA PCR Primer, Index 17 (100% over 43bp)
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC192310.22476233174527466No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA192070.22448183172125682No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT168390.19680582935149912No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC158010.18467420331272866No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGAC136340.15934738864412015No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA128650.15035970037454935No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC128440.15011426285353374No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG103440.12089551035167807No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA102930.12029944780064022No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACT100820.11783338508948361No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC93510.10928982185794099No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT93110.10882232181791132No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCT88140.1030136338205424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT34300.060.04004742
TCGTATG38450.059.03307743
GTCACGT39550.058.26238329
TATGCCG39200.057.73099546
ACGTAGA41800.055.1265732
AGTCACG42450.054.2821528
CGTAGAG42750.053.4980833
CACGTAG43450.053.1913231
GTATGCC43300.052.66259445
ACGTCTG45850.051.68964815
CACGTCT46650.050.95231214
ATGCCGT44800.050.66892647
CAGTCAC46100.049.90949227
ACACGTC49900.047.9105913
TTTTTCC81000.047.4979671
GCCGTCT48050.047.02639849
CCGTCTT48300.046.85386750
GTCTGAA51350.046.15003217
TAGAGAT50400.045.8569435
TCACGTA51900.044.7971930
Produced by FastQC (version 0.11.2)