Basic Statistics
Measure | Value |
---|---|
Filename | HGV7YBGX2_n01_4s-07_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10269050 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 16589 | 0.1615436676226136 | TruSeq Adapter, Index 19 (97% over 40bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 16509 | 0.16076462769194813 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 11661 | 0.1135548078936221 | No Hit |
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT | 11258 | 0.10963039424289492 | No Hit |
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC | 11029 | 0.10740039244136507 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 2475 | 0.0 | 54.35724 | 44 |
TCGTATG | 2920 | 0.0 | 53.397823 | 45 |
TATGCCG | 2945 | 0.0 | 52.475742 | 48 |
TGAAACG | 3015 | 0.0 | 52.4 | 35 |
CACGTGA | 3040 | 0.0 | 52.082554 | 31 |
GTCACGT | 3060 | 0.0 | 51.517193 | 29 |
AGTCACG | 3170 | 0.0 | 49.83835 | 28 |
ACGTCTG | 3295 | 0.0 | 49.205612 | 15 |
TCACGTG | 3220 | 0.0 | 49.171585 | 30 |
CACGTCT | 3595 | 0.0 | 45.291573 | 14 |
GAAACGA | 3520 | 0.0 | 44.686398 | 36 |
GTATGCC | 3530 | 0.0 | 44.170437 | 47 |
ACACGTC | 3765 | 0.0 | 43.429996 | 13 |
ATGCCGT | 3605 | 0.0 | 42.772633 | 49 |
CAGTCAC | 3805 | 0.0 | 41.974323 | 27 |
GTCTGAA | 3980 | 0.0 | 41.168774 | 17 |
TGTTACC | 3420 | 0.0 | 37.018295 | 13 |
TAGTTGT | 3535 | 0.0 | 36.985054 | 9 |
CGTATGC | 4280 | 0.0 | 36.91388 | 46 |
CCGTCTT | 4385 | 0.0 | 35.63593 | 52 |