FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_4s-07_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_4s-07_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10269050
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA165890.1615436676226136TruSeq Adapter, Index 19 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC165090.16076462769194813No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG116610.1135548078936221No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT112580.10963039424289492No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC110290.10740039244136507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24750.054.3572444
TCGTATG29200.053.39782345
TATGCCG29450.052.47574248
TGAAACG30150.052.435
CACGTGA30400.052.08255431
GTCACGT30600.051.51719329
AGTCACG31700.049.8383528
ACGTCTG32950.049.20561215
TCACGTG32200.049.17158530
CACGTCT35950.045.29157314
GAAACGA35200.044.68639836
GTATGCC35300.044.17043747
ACACGTC37650.043.42999613
ATGCCGT36050.042.77263349
CAGTCAC38050.041.97432327
GTCTGAA39800.041.16877417
TGTTACC34200.037.01829513
TAGTTGT35350.036.9850549
CGTATGC42800.036.9138846
CCGTCTT43850.035.6359352