FastQCFastQC Report
Sat 20 Aug 2022
HGV5FDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV5FDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992081512
Sequences flagged as poor quality0
Sequence length10
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGACCTA87169211487.86496910346617No Hit
GGGTGACCTA409786844.130576319015207No Hit
GGATCTCGGT258269482.603309071644105No Hit
AAGTGACCTA179106511.8053608280526046No Hit
GGGGGACCTA80354070.8099543135120938No Hit
TTGTGACCTA48082760.48466541729083246No Hit
AGGTGTCGGT24405970.2460077090923553No Hit
AGGGGGCGGG16780000.1691393277370136No Hit
GGGTGTCGGT15311880.15434094693622313No Hit
GGGGGGGGGG15284410.15406405436572634No Hit
NAGTGACCTA12278410.12376412473655693No Hit
AGGTGGCGGT10646630.10731608110040056No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)