FastQCFastQC Report
Sat 20 Aug 2022
HGV5FDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV5FDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992081512
Sequences flagged as poor quality0
Sequence length10
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAAGGGC93818297294.5671258512476No Hit
GGGGGGGGGG399706994.028973276542623No Hit
GGGCAGGGGC22632990.22813639530861454No Hit
CGGCAGGGGC20341160.2050351685215156No Hit
CGGGGGGGGG19684440.19841555116088083No Hit
CGTCAGGGGC19419040.19574036775316905No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)