FastQCFastQC Report
Tue 20 Jul 2021
HGV55BGXJ_n01_4QG3487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV55BGXJ_n01_4QG3487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589371
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTCGTAT1829893.987234852009131TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCGCGTAT261320.5694026479881448TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTCGGAT113520.24735415811883588TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTGGTAT87010.1895902510387589TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCGCGGAT80770.1759936165544254TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCGGGTAT47540.10358718002968163TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTCGTTT46520.10136465323897327TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)