FastQCFastQC Report
Tue 20 Jul 2021
HGV55BGXJ_n01_4QG3365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV55BGXJ_n01_4QG3365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4693204
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTAT2544765.4222232828575105TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGTAT248390.5292546413921065TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGAT157340.33525071571574555TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGGAT84410.1798558085265418TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTTT63030.1343005758965517TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTGGTAT51170.1090299931560614TruSeq Adapter, Index 18 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)