FastQCFastQC Report
Tue 20 Jul 2021
HGV55BGXJ_n01_4QG3355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV55BGXJ_n01_4QG3355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457716
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT912832.0477527056456712TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTAT380990.8546753539256426TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGAT173690.38963899898513055TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGTAT148670.33351160100822935TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTGGTAT139830.3136808177102354TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGGAT132530.29730471838044414TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGAT119340.2677155745229171TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTTT54680.12266371388397108TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTGGGAT53090.11909686485186584TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGGTT48760.10938337031789373TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTTT45600.10229453827924435TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)