FastQCFastQC Report
Tue 20 Jul 2021
HGV55BGXJ_n01_4QG3229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV55BGXJ_n01_4QG3229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6382281
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGGGGAT306970.4809722417424115TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGGGGGT268880.42129138469459426TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGGGGTT220090.34484536171315555TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGGGTAT150560.23590311990336998TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGAT86440.13543747133665848TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGGGTTT74200.11625937497894562TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGTAT71910.11267131610156306TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTAT66960.10491546830984094TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTGGTAT66900.10482145803357765TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)