FastQCFastQC Report
Tue 20 Jul 2021
HGV55BGXJ_n01_4QG3117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV55BGXJ_n01_4QG3117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4969506
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT1363582.7438944635543248TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGTCGTAT209630.4218326731067434TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGTAT172540.34719748804005873TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGTAT78520.15800363255422167TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGGAT54940.11055424824922236TruSeq Adapter, Index 4 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)