FastQCFastQC Report
Thu 29 Jul 2021
HGV2WDRXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV2WDRXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113862173
Sequences flagged as poor quality0
Sequence length10
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTACTCGG56348960726.65687546697019No Hit
AGGGCACGTG50562506723.919490752910125No Hit
GTGCCCGACA46322283821.913578090221115No Hit
TTAGGGTCGT42140431119.935278486105915No Hit
AGATCTCGGT682263893.227570362507263Illumina Single End PCR Primer 1 (100% over 10bp)
TTCACGCATA133792630.6329297704879268No Hit
GACTCTCCCG117851240.5575161971546383No Hit
ACCACACTAG107899790.5104390975825461No Hit
GCATTACGTA93900350.4442122632183551No Hit
GGGGGGGGGG78263330.37023856616407697No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)