FastQCFastQC Report
Thu 29 Jul 2021
HGV2WDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV2WDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113862173
Sequences flagged as poor quality0
Sequence length10
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCCCGAC55614851126.309591897881983No Hit
GCTACAAAGC49832833623.574305948848632No Hit
TTCTCGATGA45379239721.467454349494147No Hit
ACGACTACCA41867920319.80636241793424No Hit
GGGGGGGGGG797403303.7722577667791968No Hit
GCACTGAGAA135397060.6405198112223375No Hit
TCTACCATTT112910130.5341413997666535No Hit
GTAGACGAAA104622750.49493647852886763No Hit
AATGCCATGA92539360.43777385858921847No Hit
CAATCCGACA29380870.13899141758283406No Hit
GCTACAAGCA26000980.1230022483589804No Hit
TCTCGATGAA22659190.10719331794391308No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)