FastQCFastQC Report
Wed 14 Aug 2024
HGTYWDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYWDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926816247
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG747190458.061904961404935Illumina Single End PCR Primer 1 (100% over 8bp)
CCACGCGT234453812.529668753206481No Hit
TAACCGCG226362212.4423634213654433No Hit
AATCTCTC223758422.4142695029816412No Hit
CATGGCCA214462822.313973462314585No Hit
CGTAAGGA213969572.3086514796497735No Hit
TCATTACA204010292.2011945804830066No Hit
CTGACGTG182763961.9719546414036913No Hit
CCATCTTA175033821.8885493275130296No Hit
TGGCGGTC171035711.8454112188216742No Hit
AACCGTGA169933681.8335207280845176No Hit
ATGTCAAT165889351.7898839229131467RNA PCR Primer, Index 15 (100% over 8bp)
AGTTGGCT158416851.7092584480772486No Hit
GGAGTTCC149884081.6171930572555016RNA PCR Primer, Index 1 (100% over 8bp)
ACCTAGTA138470391.4940436191986608No Hit
GTCTGAGG137482511.4833847641861635No Hit
TTCCACGA127474221.375399065484876No Hit
TACTCCTT125118331.3499798952056998ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
TCAGGCAA124837651.3469514631846975No Hit
TATGCAGT117057311.2630045101054426No Hit
ATAGAGAG113483791.2244475684078076ABI Solid3 Adapter B (100% over 8bp)
TTCCGTCT109461811.1810519113612388TruSeq Adapter, Index 14 (100% over 8bp)
AGAGGATA104456261.1270439025870895No Hit
CCAATGCT103859171.1206015252341601No Hit
TCTACTCT102936271.110643780071758No Hit
TTCTTCCG101360051.0936369569274502No Hit
CTGTTGGA101322651.093233424942323No Hit
TCTCAGTG98509081.0628760589692166No Hit
TTCTCTGC95964201.0354177574101158No Hit
CACGACAT95122661.0263378561597443No Hit
GCATCCAT95056691.025626064580631No Hit
GGTACCTT94498891.0196076116045902No Hit
CAGTTCCT93459401.0083919040318678No Hit
CGATCTCG93277251.0064265737887954Illumina Paired End PCR Primer 2 (100% over 8bp)
CACTCACG92231600.9951444021244051No Hit
TTGGTGCT92062150.9933161001222716No Hit
GTTAGTGC90038000.9714762801304236No Hit
CTCCTTAC88059840.950132675004779No Hit
GCTCACAA86800600.9365459472788029No Hit
TGAACCGA83880140.9050352782605029No Hit
TGATCTCG81692570.8814322177069043Illumina PCR Primer Index 5 (100% over 8bp)
ATGCCTCA80659430.8702850242546514No Hit
GCGATCTA78159580.8433125795214939No Hit
AGATATCC77894000.8404470708420804No Hit
TTCAGCAC77532440.8365459739291774No Hit
GAGTAGCC77281420.8338375621937063No Hit
TGTTACGG75968590.8196726184494691No Hit
AGGCTTAG73747730.7957103712706063No Hit
TTCACGCA72650050.7838668154033773No Hit
CTCGATGA72627490.7836234014572686No Hit
CACCTACA72611960.7834558385768134No Hit
TCCAGAGA71713300.7737596339309749No Hit
GCATTGTC71528030.7717606400570576No Hit
GAGGCTAT70444290.7600674915661033No Hit
CCTGAACT70435460.7599722191749623No Hit
GGGGGGGG68239890.7362828416191975No Hit
CAGATAGT66089360.7130794287856285No Hit
CTCTAGCT62197720.6710900914968532No Hit
CGTACGAT61114930.659407193149906RNA PCR Primer, Index 22 (100% over 8bp)
TGCAACCT60150040.6489963916223838No Hit
TTATGGCC58494360.63113222485406No Hit
GGACGTAT57175450.6169016801881765No Hit
GGCTAACA57097790.6160637578896477No Hit
ACGGTGTC56491190.6095187712003931No Hit
ACTCGCTA55761000.6016402947239228No Hit
GTTCCTAG55623810.6001600660330245No Hit
ACACGCAG54584850.5889500769616958No Hit
GAAGGACT52395760.5653306161776855No Hit
GTCGGTAA49570490.534847011588911No Hit
GGTCTATG49434590.533380701514612No Hit
TAGCACAG47583890.5134123420259809No Hit
GACTTCGA47079810.5079735077194866No Hit
TGTTCTCG41598050.4488273714951395No Hit
GACCATTG39871370.4301971413325904No Hit
GTTAACCG39410420.42522366356402463No Hit
GAAGTCAG36336600.39205829761419797No Hit
TGGTTCAC31455860.33939694197009473No Hit
CTCGTCGA20552390.2217525865189111No Hit
TAGTAACG17249160.1861119726357149No Hit
GTGACTCT15709740.1695022077013719No Hit
GGTTCTCG15491700.16714963780733116No Hit
TATCTGAC15365920.16579251874077255No Hit
AGTGTAGA15195110.16394954284827076Illumina Single End PCR Primer 1 (100% over 8bp)
CCTGGCCA14934490.16113755071020028ABI Solid3 GAPDH Forward Primer (100% over 8bp)
ACACGATC14027990.15135675540223886No Hit
TATAGTAG13825740.14917455369122376No Hit
GGATCTCG13645140.14722594736732103RNA PCR Primer, Index 23 (100% over 8bp)
GTAGATCG12647700.13646394353723496No Hit
CGAGACGT12193010.1315580088228643No Hit
CACTAGAC12184380.1314648943567775No Hit
TGCTGCTT11962460.12907046071668615No Hit
GTCTCGTA11477060.1238331766102499TruSeq Adapter, Index 14 (100% over 8bp)
CATACTAG11222050.12108171426994849No Hit
ACGCTATC10168010.1097090176495363No Hit
GGGTCTCG9999750.10789355530147499No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)