FastQCFastQC Report
Wed 14 Aug 2024
HGTYWDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYWDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926816247
Sequences flagged as poor quality0
Sequence length8
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGG11909500712.849904971508339No Hit
AGCCATGC256487792.767407140630326No Hit
CCTAAGAC250721002.705185637515049No Hit
ACAGATTC243274842.6248443614087833No Hit
ACTGAGCG227261342.4520646971351594No Hit
CGAGGCTG225036202.428056270360137No Hit
GCTACGCT221333112.3881013169161673No Hit
ACTCGCTA218050842.352686853578647No Hit
CGATCAGT206189262.224704850259277No Hit
AGCACCTC196101162.1158580315651285No Hit
ATCATTCC190324502.053530034848429No Hit
ACACGACC186223352.0092801631691724No Hit
TGGCTTCA185312661.9994541593313264No Hit
TAGCGCTC184564581.9913826564587618No Hit
GCTCATGA181000291.952925303002376No Hit
AATCCGTC176673731.906243341890833No Hit
CAGAGAGG174449941.882249481109927No Hit
AAGGACAC171274901.847991989290192No Hit
TCTTCACA163944451.7688991807240082No Hit
TTCACGCA163379251.762800884520964No Hit
CTCTCTAC161969801.7475934471830639No Hit
ATCTCAGG159402251.7198905448190744No Hit
TATCAGCA142559141.5381596995245594No Hit
TCCGTCTA133872461.4444336774773867No Hit
ATGCGCAG133708371.4426632078667045No Hit
CGGAGCCT129079601.3927205141020795No Hit
TACGCTGC123306991.3304362153677265No Hit
GGAGCTAC123287061.3302211781360798No Hit
TAGGCATG116924071.2615669004343641No Hit
CTCGTTGT112823401.2173222077752377No Hit
GCGTAGTA109596181.1825017133088735No Hit
AAGAGGCA109184161.178056171904807No Hit
GGACTCCT108309261.1686163287554021No Hit
GCGTATAC104741321.130119593166778No Hit
GTAACGAG102325931.1040584401839904No Hit
CGAAGTAT102175311.1024333068257057No Hit
AGGCAGAA94223721.01663863041883No Hit
CTCGACTT92518720.9982423193321512No Hit
TGCTCGTA85265850.9199865698944744No Hit
TCCTGAGC85079910.9179803469716258No Hit
CTAATGGC82683110.892119773122622No Hit
CCTTGAAG82406590.8891362259427462No Hit
CGTACTAG81144100.87551443193464No Hit
CACCTATC76618080.8266803721665876No Hit
GTCTTGTC74832580.8074154962456113No Hit
ACCAAGGA74589160.8047890856621982No Hit
TAAGGCGA72365900.7808009433826855No Hit
GGTAACGT67771170.7312255284622777No Hit
TTGGAGAG66364560.716048733660147No Hit
GAATCCAG66032950.712470786024104No Hit
CAACAGAG65954720.7116267136391707No Hit
GATCTACG63557430.6857608528737844No Hit
GCCATACA59438950.6413239969885854No Hit
GTAGAGGA51758220.5584517984825529No Hit
TCATTGCG45650970.4925568595475862No Hit
CAACCGAG20491620.22109690099120588No Hit
CTACGACC19953440.2152901404629779No Hit
TAACGTCC19752630.21312347581235272No Hit
GTTACAGC14474220.15617140988681869No Hit
ATAGTACC12961330.13984789371090944No Hit
ACGTGCGC11927800.12869649230480096No Hit
TAGCAGCT11777920.12707934326921655No Hit
CGCGATAT11212530.12097899703737067No Hit
ACGTACGT11079540.11954408477260973No Hit
AACGCTGA10803830.11656927718920317No Hit
GCGACTAA9323090.10059264746574949No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)