FastQCFastQC Report
Fri 15 Mar 2019
HGTYHAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYHAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27623676
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380620713.778785271011722No Hit
CATCTGACGCTGCCGACGACGGAGAGAGTGTAGATCTCGGTGGTCGCCGT348520.12616713286095596Illumina Single End PCR Primer 1 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA50450.034.219972
CGATCTG853500.024.6462975
GATCTGG339800.024.4360476
ATCTGCG89500.022.0980537
ATCTGGG163300.021.3501917
CCGATCT2127450.020.0134124
CGTATCA146050.019.65041248
GCCGTAT144850.019.64463646
TTCCGAT2178400.019.5828042
TCCGATC2205550.019.3701153
TAGTGTA35250.019.35598670
GATCTGA245000.019.2174536
GTATCAT152750.018.78842549
CCGTATC152850.018.61615447
CTTCCGA2307900.018.546061
GATCTGC299700.018.4431486
CGCCGTA157400.018.25669545
CTAGGCG10150.017.932179
AAGGGGG161150.017.74038565
GAGTGTA151200.017.38583226
Produced by FastQC (version 0.11.5)