FastQCFastQC Report
Fri 15 Mar 2019
HGTYHAFXY_n02_r4zt2s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYHAFXY_n02_r4zt2s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114.285714285714285No Hit
GCACACGTCTCCCAGCGTAGATCGGAAGAGCACACGGCTCGCGGGGAAGA114.285714285714285Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGAAACAGTAACAAAAAGGCTACAGAAAAACCATCTCACAAACTAAATT114.285714285714285No Hit
AGACGTATGCACAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114.285714285714285No Hit
ACTAAACAAAATAATCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114.285714285714285No Hit
CTTCCGAACAAGAAGCGGAAGAAATTAGGAAAACATCCATGAAAACTACC114.285714285714285No Hit
GTTTATATGGCAGACATAATTTTTTACTTGTTTAATACTTACTGATATGA114.285714285714285No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)