FastQCFastQC Report
Fri 15 Mar 2019
HGTYHAFXY_n02_r4zt2s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYHAFXY_n02_r4zt2s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGATGTAGGTCGGGACTTATGTACCCTGTCTCTTATACACATCTGACG17.6923076923076925No Hit
AATGAAAGAAACTAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
AAGAGACGAGTACTCTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
CTCAAATAATGACCCTATTGCGGTTTCACATTTAATTCGTGTGTACTTAG17.6923076923076925No Hit
AAACAAAACGAGAAATGCTCTCCGAAAAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
AACAAACAAAAAAAATACTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
TCCGATCTACACAGAATACTCTACCAAAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
CATACAATCTATGCCGAAATACTCTTCCGAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
AAACCAATCAACACAAAAAACTCTACCAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
CATACATGCTTCACATGACGTCTACGCGAACATCCTTCAGGACAATCACG17.6923076923076925No Hit
GATCTGGACCGGGAGACGTGTGCTCTTCCGATCTGACTTGGGAGACGTGT17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCAAACAGACTAAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17.6923076923076925No Hit
CCATTAGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)