FastQCFastQC Report
Fri 15 Mar 2019
HGTYHAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYHAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27623676
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295090510.682521037388362No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT527000.1907783743191891No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT474960.1719394623655447No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT418320.15143531222998705TruSeq Adapter, Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT378800.13712874419754995RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG340600.12330002712166188TruSeq Adapter, Index 6 (96% over 28bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG284610.10303118238137458RNA PCR Primer, Index 41 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA62650.032.127362
CGATCTG871800.023.6275845
GATCTGG353100.023.4137576
GATCTTT345200.022.36385311
ATCTGGG172350.020.9140367
CGATCTT555350.020.3638525
ATCTGCG87700.020.3109447
CTCGTAT1055350.020.16941339
ATGCCGT1072300.020.04468344
CCGATCT2424550.019.997254
AAGGGGG1072100.019.8812669
TCTCGTA1073850.019.79590238
GCTTGAA1084850.019.73493856
AAAGGGG1086800.019.69521368
TTCCGAT2470100.019.6713662
GATCTGA239050.019.6641986
AGGGGGG1084650.019.66165470
CTTGAAA1097800.019.54007757
AAAAAGG1110550.019.52033666
TTGAAAA1102400.019.50933858
Produced by FastQC (version 0.11.5)