FastQCFastQC Report
Fri 15 Mar 2019
HGTYHAFXY_n01_r4zt2s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTYHAFXY_n01_r4zt2s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGCGC17.6923076923076925No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCGAAAACCA17.6923076923076925No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTGGGAACGGGACCCGGCGCCCC17.6923076923076925No Hit
TTGCAGAACGGTTCACAGCCTGAACATTTGATGCGTTTGCACAATCCAAT17.6923076923076925No Hit
GGTACATAAGTCCCGACCTACATCTTCCTGTCTCTTATACACATCTCCGA17.6923076923076925No Hit
CTACCGATCACATGGCGCAGACGAGAGCTCATCCGAACTCGCAACGGAAT17.6923076923076925No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17.6923076923076925No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAAGAC17.6923076923076925No Hit
CTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTACCCCCCACCCAAA17.6923076923076925No Hit
TTATAGATCACCTAGCAATTATCGTCTAGCGTGATATAGCCGTGCGCGAA17.6923076923076925No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG17.6923076923076925No Hit
CGTCTCCCTCAGAAGATCGGAAGAGCACACGTCTCCCAAGTCAGATCGGA17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTAA17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)