FastQCFastQC Report
Wed 7 Jul 2021
HGTNGBGXJ_n01_VvMYB135_PN94-B_s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTNGBGXJ_n01_VvMYB135_PN94-B_s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407829
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT24080.590443543740146TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA9610.23563797572021608TruSeq Adapter, Index 27 (97% over 39bp)
GGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAG7350.18022259329277712Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)