FastQCFastQC Report
Wed 7 Jul 2021
HGTNGBGXJ_n01_VvHYH_ampPN94-B_s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTNGBGXJ_n01_VvHYH_ampPN94-B_s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226519
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGG5170.22823692493786396No Hit
AGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTA4110.18144173336453012No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG3310.1461246076488065No Hit
CCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACC3130.1381782543627687No Hit
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC2870.1267001885051585No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2850.12581726036226543TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)