FastQCFastQC Report
Wed 7 Jul 2021
HGTNGBGXJ_n01_MdPS90_Md-B_s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTNGBGXJ_n01_MdPS90_Md-B_s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195360
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT14360.735053235053235TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA8170.41820229320229324TruSeq Adapter, Index 2 (97% over 37bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGT4660.23853398853398855TruSeq Adapter, Index 2 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGT4380.22420147420147418TruSeq Adapter, Index 2 (97% over 37bp)
GGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAG3510.17966830466830466Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)