FastQCFastQC Report
Wed 7 Jul 2021
HGTNGBGXJ_n01_AtTGA5_Md-B_s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTNGBGXJ_n01_AtTGA5_Md-B_s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14620229
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1174080.8030517169053918TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA572640.39167649152417516TruSeq Adapter, Index 3 (97% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGT269400.18426523962107572TruSeq Adapter, Index 3 (97% over 36bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGT257090.17584539886481942TruSeq Adapter, Index 3 (97% over 36bp)
GTTCAAAATGACCTTATCAAACATAGAAGGATGCAGAAACTTGAATTTCT224960.1538689989055575No Hit
GGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAG167770.11475196455541155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)