FastQCFastQC Report
Wed 7 Jul 2021
HGTNGBGXJ_n01_AtSPL16_ampCol-B_s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTNGBGXJ_n01_AtSPL16_ampCol-B_s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83435
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4380.5249595493497933TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC3740.44825313117996046TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)