FastQCFastQC Report
Thu 23 Sep 2021
HGTMKDRXY_n01_dei074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTMKDRXY_n01_dei074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32181593
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT15284564.749472780915475TruSeq Adapter, Index 20 (97% over 44bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8256632.5656374437399663No Hit
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4082621.2686196112168842No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT2685780.8345702464138429TruSeq Adapter, Index 20 (97% over 45bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1191360.3701992005181347No Hit
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG668720.20779580426612196No Hit
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG572780.17798373125904612No Hit
TCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG364320.1132075717942241No Hit
ACGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG359160.11160417074443767No Hit
AGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG341610.10615074275533844No Hit
ATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323520.10052951698195922No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)