FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_sb303_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_sb303_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41855141
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAG499300.038.064431
TGAGGTA137100.030.8433744
TTTGAGG331850.026.673452
GTAGATA226050.022.457233145
GAGGTAA122750.022.2786335
GTAGCCT85850.019.8415581
AGTTTGA422250.018.8580133
GGGTAAT48650.018.6241041
CAAGTTT471250.018.3654331
GGTTAAT107900.017.8693351
AGGTAAT82350.017.4412066
AGCCTTG96300.017.2453673
AAGTTTG493650.016.6679442
ATAGCAT112000.016.5805728
GACTAGG55350.014.5472479
TAGCCTT143500.014.4536443
GTAAAAA179550.014.3718541
TTGAGGT355800.014.3696573
TAGCATT131750.013.9848759
TTTGAGC212950.013.3108072
Produced by FastQC (version 0.11.2)