FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5174rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5174rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51799375
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAG668900.034.9480971
TGAGGTA167950.029.6259254
TTTGAGG427250.021.2775542
TTGAGGT312550.018.7709883
GAGGTAA236650.017.9621525
GGGTAAT101250.016.8955381
GTAGATA258850.016.865847145
AGAGCGG206550.016.396513145
TCCGTCC219450.015.833367
CCGTCCC224000.014.7345528
CAAGTTT814500.013.8742671
AGGTAAT168650.013.0324886
GGTAATA133200.012.2457262
AGTTTGA949500.010.93723
GCGGTAG96000.010.80424
AAGTTTG999250.010.5123652
GTAAAAT505650.010.2927041
CCGTATA102700.010.097769145
GAGGGTA103050.09.8547895
TGAGGGT152200.09.8170364
Produced by FastQC (version 0.11.2)