FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5173_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5173_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46933086
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA152650.043.6665534
GTTTGAG659850.034.8330271
TTTGAGG374650.027.4573972
GAGGTAA227750.025.9262245
TTGAGGT300750.024.4508743
GTAGATA226450.022.707014145
AGGTAAT128200.019.4082686
CCGTCCC137100.016.8785748
AGAGCGG228250.014.425511145
CCGTATA98950.013.55945145
TCCGTCC199300.012.6663977
CGGTGGG84100.012.590501145
GGGGCAC58600.012.3789029
AGGTAAA199200.011.8351766
CTGGGGC88650.011.5377617
CAAGTTT951650.010.8765491
TTTGAGT437100.010.2165112
TGAGGGT192450.010.1017837
AGTTTGA895550.010.08188153
TGGGGCA79400.09.8670158
Produced by FastQC (version 0.11.2)