FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5112_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5112_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37789545
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA74700.043.5972484
GTTTGAG418850.030.7173141
GTAGATA180900.026.01786145
GAGGTAA123400.024.8653625
TTTGAGG276700.020.90752
TTGAGGT199650.018.0896953
CGGTGGG54850.015.072791145
CCGTATA61800.014.90324145
AGGTAAT99750.014.6170676
AGAGCGG169150.014.44852145
GGGTACC20550.013.0506841
GGGTACT57400.012.75418951
GGGTATA43350.012.7077271
GGGTAAT70000.012.4258641
AGTGTAT156850.012.298796145
CGGTCCG51550.012.2424647
TGAGGGT98200.011.8179424
GGTCCGG54600.011.6914788
TCTCGGG69100.011.439681145
AGGTAAA147200.011.3343646
Produced by FastQC (version 0.11.2)