FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5070_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5070_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47201741
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA93900.044.5699044
GTTTGAG434600.036.0917131
TTGAGGT195500.023.6298053
TTTGAGG308900.022.436042
GTAGATA237850.022.258856145
GAGGTAA201600.019.8620175
TAGTGCG66450.017.1391458
GGTCCGG63450.017.0348828
CTAGTGC68400.016.544447
CCGTATA70900.015.9573765145
CCGGTCC76100.015.15638356
CGGTCCG74050.014.4984047
GGGTACC34700.014.4131721
CCCGGTC74700.014.3719585
AGAGCGG187550.014.191624145
GGGTACT47500.013.7337411
ACCCGGT80050.013.5913134
TGAGGGT79250.012.6302324
CGGTGGG78700.012.072013145
TCTCGGG66800.011.834043145
Produced by FastQC (version 0.11.2)