FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5025_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5025_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39353076
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAG462600.038.954341
TGAGGTA167000.033.3103374
GGGTAAC153850.022.615391
TTTGAGG407050.021.6748282
GAGGTAA212450.018.8803445
CAAGTTT444800.018.0239811
TAGGGCC46350.017.057948
CTAGGGC45800.016.9460147
GCGTCGG223600.015.956986145
AGTTTGA510300.014.75085453
AGAGCGG352600.014.49988145
AAGTTTG543600.013.9897152
TTGAGGT443200.013.8916353
GTAGATA333850.013.533004145
AACGTAC147600.013.2684135
AGGTAAT148100.012.8809626
GTAAAAT220650.012.7792691
TTTGAGA476750.012.2714432
GAATTTA293700.012.1742744
TTTGAGT243950.011.9464272
Produced by FastQC (version 0.11.2)