FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5022_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5022_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46640550
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAG783600.031.5527381
TGAGGTA330500.021.5723784
GAGGTAA294550.018.9869255
GTAGATA388600.018.866793145
TTTGAGG753900.017.8277322
CAAGTTT734950.016.5790521
AGAGCGG337150.016.088968145
AGTTTGA757950.014.7056573
CGGTGGG137550.014.498458145
AAGTTTG832200.013.9116072
TTGAGGT635150.013.6440323
AGGTAAT185500.013.09983356
AGGTAAA237750.012.1430416
GCGTCGG329300.010.746767145
GGGTAAT149200.010.4938461
TAGGTCC56450.09.249533
TTTGAGA725900.09.0579642
TCTCGGG207200.08.64484145
AAACATG474800.08.5850254
CCAAGTT919800.08.4715881
Produced by FastQC (version 0.11.2)