FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5020_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5020_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46011248
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAG399050.032.3140641
TGAGGTA162000.022.162564
AACGTAC116050.021.189735
TACCTAA115050.020.5545739
CAACGTA133900.017.984044
GTAGATA266300.017.783604145
ACGTACC149200.016.238976
GTACCTA155450.015.9127558
TTTGAGG406550.015.3886242
CAAGTTT380100.014.9506871
GGTAAAA166850.014.9008441
AAGTTTG371350.014.6218162
CGTACCT164950.014.60049257
CCGTATA81750.014.016743145
AGAGCGG532750.013.245463145
GGGTATA65550.013.056874
CGGTGGG174400.012.558513145
GAGGTAA207850.012.2497715
AGTTTGA458950.011.8036663
TTGAGGT330800.011.6410113
Produced by FastQC (version 0.11.2)