FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n02_df5010_rna_seq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n02_df5010_rna_seq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42306392
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAG461900.034.3676761
GTAGATA318100.025.921303145
TGAGGTA180750.018.3783864
CGGTGGG110000.017.207108145
CAAGTTT494050.016.2271
AGTTTGA479550.014.8957863
TTTGAGG512750.014.2799852
CCGTATA144700.013.231085145
GAGGTAA191850.013.0443285
AGAGCGG336100.013.032522145
GCGTCGG253050.012.839015145
TCTCGGG151100.012.718663145
TTGAGGT351450.012.0506483
CTCAATC452050.011.6589662
AGTGTAT271250.011.416112145
AAGTTTG649050.011.3928952
GGGACAT134800.011.2385531
ACTTAAG204350.09.7617246
CGCCGTA696250.09.561753145
TTTGAGA628700.09.4900482
Produced by FastQC (version 0.11.2)