FastQCFastQC Report
Thu 13 Sep 2018
HGTGFBGX7_n01_sb303_rnaseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGTGFBGX7_n01_sb303_rnaseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41855141
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTATT302150.015.0933721
TTTAATC73150.014.3697642
GAGCACC563800.014.338265145
AAGAGCC480900.013.94549145
TTTTAAT189900.013.5919971
CCAGTCC443600.011.620502145
TTTTTAA279850.011.1145081
TATGCCG527200.011.056749145
TTTATTT262700.010.9001632
CAATATG261100.010.829403145
TTTTTTA314100.010.7335461
CTTTATT263050.010.0327811
TTTATTC414450.09.8476412
CGTATGC731500.09.832051145
CAATTTA241850.09.6213884
TCGTATG770850.09.433609145
TTTAATG62150.09.3313572
GTATGCC626900.09.286757145
GCCAATA1352600.09.064024145
ACGCCAA1589350.09.032178145
Produced by FastQC (version 0.11.2)